Math Biology Seminar: Gautam Menon
Topic
Modeling cell-substrate de-adhesion dynamics under fluid shear
Speakers
Details
Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. I will describe theoretical and experimental investigations of the detachment of cells adhered to substrates when these cells are subjected to fluid shear. I will present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as for the decay in the fraction of detached cells at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, characteristic force scales for cell adhesion as well as characteristic detachment times are extracted. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis.
Additional Information
Location: ESB 4127
Gautam Menon - IMSC, Chennai
Gautam Menon - IMSC, Chennai
This is a Past Event
Event Type
Scientific, Seminar
Date
June 27, 2018
Time
-
Location